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If you need to get a file in one format to another, such as RAW to JPEG, there are a number of free applications out there that will do it for you. One of the most popular open-source applications is The Gimp, available for download at www.download.com, a great site for finding many types of applications. The Gimp is available for Windows, Mac OS X, and Linux. In addition to The Gimp, you can also take a look at your digital camera s software package it probably came with a photo conversion program such as Adobe Photoshop LE, Adobe Photoshop Elements, Paint Shop Pro, ArcSoft PhotoStudio, Ulead Photo Impact, or one of many other commonly bundled applications.

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Option or Alt for the Page Grabber Hand is missing. Use the quick zoom function instead, as described later in this chapter. +K or Ctrl+K for deleting selected objects is missing. You can always use the Delete key unless the Type tool is selected. That s because even though InDesign s QuarkXPress 4.0 shortcut set maps the Preferences dialog box to QuarkXPress 4.0 s +Y or Ctrl+Y shortcut, it also retains the standard Adobe shortcut of +K or Ctrl+K to open the Preferences dialog box.

B C D F G H J K L M N P Q R S T V W X Z # # # # # # # # # # # # # # # # # # # # t v n w c k z h b d r p

output as spot colors or are color-separated into CMYK. Other models (such as Toyo, ANPA, and DIC) may or may not separate accurately for all colors, so check with your printer or the ink manufacturer.

A baseline grid is document-wide (that is, you can t change it from page to page), and grid lines are displayed behind all objects, layers, and ruler guides. The default baseline grid begins 3 picas (1 2 inch) from the top of a document page; the grid lines are light blue and placed 1 pica apart; and they appear at view percentages above 75 percent. If you change any of these settings when no documents are open, the changes are applied to all subsequently created documents; if a document is open, changes apply only to that document.

4.3.4. Gelatin-Coated Flasks, Plates, or Dishes (i) Gelatin 0.1%: dissolve 0.5 g gelatin in 25 mL of distilled H2 O and autoclave. Add the hot gelatin solution to 475 mL of distilled H2 O and store at room temperature. (ii) Add to asks, plates, or dishes at 1 mL/cm2 , leave 5 min at room temperature, and remove. (iii) Allow to dry overnight in sterile laminar ow and store at 4 C until required. 4.4. PROTOCOLS FOR HESC DIFFERENTIATION TO CARDIOMYOCYTES The cut-and-paste method has been reported to be the most supportive of karyotypic stability, but most hESC lines can be adapted to bulk culture methods (trypsinization, collagenase) and can be cultured on mouse embryonic broblasts (MEFs; see Protocol 4.4) or human feeder cells (e.g., foreskin broblasts). In our hands, hES2 and hES3 cells are passaged routinely once a week on MEFs. At passage, colonies are usually 0.5 2 mm in diameter but will not become con uent.

In Listing 6-5 is a barebones HTML page that will serve as the launch pad for mapping geotagged del.icio.us bookmarks. Notice that one of the first things appearing in the <head> of this page is a JavaScript include using the Google Maps API key address. You ll want to supply your own here, or else you ll receive a few JavaScript alerts when you try to load up this page. Next in the page is a reference to a JSON feed of my bookmarks filtered by geotagged. This will pull in all of the bookmarks I ve posted that include geographical metadata. Swap deusx for your own user name to base this on your own collection. The next two elements in the page header make reference to CSS and JS code, which you ll see defined very shortly. Finally, the <body> of this page simply includes a title, a <div> with an ID of map, and an unordered list with an ID of legend. The latter two elements are empty for now, but they will soon be filled with content on-the-fly via JavaScript. Listing 6-6 offers the CSS included for this page as a reference to ch06_gmaps_geotags.css.